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Amino Acid

L-Proline

Also known as: Proline, Pro, L-Pro

C
Evidence

L-Proline is a non-essential amino acid that serves as a structural component of collagen and elastin, and is involved in protein synthesis and tissue repair. It has limited direct clinical evidence as an isolated supplement, though adequate proline intake supports connective tissue health.

Primary uses

  • Collagen synthesis support
  • Joint and connective tissue health
  • Skin health and elasticity
  • Protein synthesis
  • Wound healing support

How it works

  • Acts as a structural amino acid in collagen (comprises ~10% of collagen)
  • Substrate for hydroxylation to form hydroxyproline, critical for collagen cross-linking
  • Participates in general protein synthesis

Dosage

Typical range
1,500–5,000 mg daily
Timing
Anytime; often combined with other collagen-supporting amino acids (glycine, hydroxyproline)
With food
May be taken with or without food
Duration
Ongoing use typical for structural support; effects on skin and joint health may require 8–12 weeks
Special populations
Adequate dosing may support recovery in athletes; individual needs vary based on dietary protein intake

Forms

  • Powder (free amino acid)· 70/100

Safety

Common side effects

  • Minimal; occasional mild GI upset at high doses

Evidence notes

L-Proline is biochemically essential for collagen formation with strong mechanistic support, but isolated supplementation lacks robust RCT evidence demonstrating superior outcomes versus dietary protein intake. Most evidence is derived from collagen peptide studies that include multiple amino acids. Direct proline supplementation studies are limited.

Grade C: Mostly observational or small trials; mechanism is plausible but unproven at scale.

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Medical disclaimer. This page is educational and does not replace advice from a qualified healthcare provider.