Amino Acid
N-Acetyl L-Glutamine
Also known as: N-Acetyl Glutamine, NAG, NAGL, Acetyl-L-Glutamine
N-Acetyl L-Glutamine is an acetylated form of the amino acid glutamine that may support intestinal barrier function and immune health. Evidence for human supplementation is limited and primarily preliminary.
Last reviewed June 2026 · Evidence graded from peer-reviewed research, not editorial opinion. Educational only — not medical advice.
Primary uses
- Intestinal barrier support
- Gut health
- Immune function
- Post-exercise recovery
How it works
- Provides acetylated glutamine for intestinal epithelial cell metabolism
- May support tight junction integrity in the gut barrier
- Potential glutamine precursor for immune cell function
Dosage
- Typical range
- 2-5 grams daily
- Timing
- Can be taken any time; some suggest post-workout or between meals
- With food
- Can be taken with or without food
- Duration
- Optimal duration for supplementation is not well established
- Special populations
- Safety in pregnant/nursing women and children not well studied
Forms
- Powder· 70/100
- Capsule· 70/100
Safety
Common side effects
- Generally well-tolerated
- Mild gastrointestinal upset reported occasionally
- Nausea possible at higher doses
Contraindications
- Not recommended for those with glutamine sensitivity
- Caution in severe liver or kidney disease
Evidence notes
Most human evidence is anecdotal or derived from animal studies. Very few robust RCTs in humans specifically examining N-Acetyl L-Glutamine supplementation. Glutamine itself has better evidence, but the acetylated form lacks sufficient clinical validation.
Grade D: Primarily pre-clinical or anecdotal; human efficacy not established.
Related in Amino Acid
Check a full stack
Formulate's free interaction checker lets you paste in any combination of supplements and medications at once — every pairing flags severity, timing, and cited evidence.
Open the checkerWhere does N-Acetyl L-Glutamine fit in your stack?
Answer two questions and get a stack graded on research — every ingredient scored on the evidence behind it, not the marketing.
Build my stack2 questions · free · no account needed to start
Also on iPhone — get the free app
Evidence-based supplement guides, no spam
We send a short digest when new guides drop — nothing else. No affiliate pushes, no sponsored content, unsubscribe anytime.
Medical disclaimer. This page is educational and does not replace advice from a qualified healthcare provider. Individual needs and risks vary — consult a professional before starting, stopping, or combining supplements, especially if you are pregnant or nursing, taking medication, or managing a health condition.